Urine levels of CCL2 and CXCL10 chemokines as an effective and non-invasive tool for monitoring the function of a transplanted kidney

The aim of the project is to determine whether urine concentrations of CCL2 and CXCL10 chemokines may be an effective, non-invasive tool for monitoring the function of the transplanted kidney and constitute an early marker of acute rejection in renal transplant patients. The project will be implemented at the Clinical Department of Transplantation Medicine, Nephrology and Internal Diseases of the Medical University of Warsaw. The study will include 50 patients who have undergone kidney transplantation. CCL2 and CXCL10 concentrations will be determined by enzyme immunoassay ELISA on frozen urine specimens collected 3 and 12 months post-transplant in the time prior to protocol biopsy. The relationship of the concentrations of the CCL2 and CXCL10 chemokines in the urine with the histopathological changes in the material obtained by protocol biopsy as well as with the factors of increased immunological risk (subsequent transplant, PRA> 80%, the number of HLA incompatibilities) and their change depending on the time after transplantation will be investigated. It will be determined whether CCL2 and CXCL10 chemokine levels could be an effective and non-invasive tool for monitoring transplant function to identify a group of high-risk patients after kidney transplant surgery, resulting in improved long-term outcomes, including better patient survival.