Molecular mechanisms and markers of vulvar squamous cell carcinoma progression

The aim of this study is to identify prognostic protein markers for vulvar squamous cell carcinoma (VC). Computerized analysis of changes at the level of regulatory pathways to model carcinogenesis could provide new strong hypotheses about the biological causes of VC progression. We intend to develop a method for quantifying the expression of a selected panel of proteins using the MRM technique (multiple reaction monitoring). The developed test will enable the quantification of the expression of the studied proteins. Correlation of the level of peptides in tumors with clinical data obtained during the observation period will allow the assessment of the predictive value of the test. The research methodology proposed in this application may lead to the detection of many proteins with different expression in tumors obtained from patients with progressive VC ("progVC") during the follow-up period compared to the expression in tumors of patients with long disease-free survival ("d-fVC" ”) During 8-12 years of observation. These proteins can then be grouped according to common biological characteristics, revealing the changes that cause the tumor's aggressive phenotype.