Development of an innovative method of treating Epidermolysis Bullosa and chronic wounds of other origin with the use of biological dressing made of human material

The proposed project is the world's first research project based on the use of an allogeneic matrix of human ADM skin inhabited by allogeneic stem cells and a dressing with autologous patient cells collected from reverse mosaicism sites in the treatment of epidermolysis bullosa. The usefulness of a novel ADM dressing filled with allogeneic stem cells will remain
also approved for the treatment of deep burn wounds and leg ulcers, where it will act as a skin graft replenishing tissue defects and as an active dressing enabling epithelization of the wound. Research tasks are assumed: 1. Creating a human scaffold; 2. Production of dressings; 3. Development of a method of sterilization of biological dressings made of human material; 4. Development of a clinical trial protocol and conducting patient qualification and recruitment for research; 5. In vitro studies of the scaffold with inhabited stem cells; 6. Clinical studies of the effectiveness of biological dressings made of human material on 100 patients; 7. Studies on reverse mosaicism in patients with EB; 8. Analysis of adhesive molecules of the dermal-epidermal border and proteins involved in the wound healing process in skin sections taken from healed sites. In the final phase of the project implementation, preparation for the implementation of an innovative product will take place.