The influence of iron and zinc on the formation of extracellular neutrophilic traps (NETs)

Aim of the project: The aim of the project is to evaluate the influence of iron and zinc on NETs ejection. In addition, by examining net ejection in patients with iron deficiency anemia and haemochromatosis (a disease characterized by the accumulation of iron in the body), I will assess whether the deficiency and excess of the test metal affects the ability of neutrophils to release the net and the efficiency of killing pathogens within the NETs. For the last stage of the research, I will include mice with haemochromatosis and mice fed with poor fodder and saturated with zinc and iron. Based on the results of research in a mouse model, I will try to explain whether a diet low in micronutrients or excessive supplementation of these elements may adversely affect the mechanisms of innate immunity. Expected results: The results of our research will contribute to a better understanding of how homeostasis of selected metals influences granulocyte function, including NET ejection. More importantly, we will estimate the effect of excessive micronutrient supplementation on non-specific immunity.